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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
06/12/2019 |
Actualizado : |
20/04/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
MAYA, L.; MACÍAS-RIOSECO, M.; SILVEIRA, C.S.; GIANNITTI, F.; CASTELLS, M.; RIVERO, R.; CRISTINA, J.; GIANNEECHINI, E.; PUENTES, R.; FLORES, E.F.; RIET-CORREA, F.; COLINA, R. |
Afiliación : |
Laboratorio de Virología Molecular, CENUR Litoral Norte Sede Salto, Universidad de la República, Gral. Rivera 1350, 50000 Salto, Uruguay; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Laboratorio de Virología Molecular, CENUR Litoral Norte Sede Salto, Universidad de la República, Gral. Rivera 1350, 50000 Salto, Uruguay.; DILAVE Miguel C. Rubino, Laboratorio Regional Noroeste, Casilla De Correo, 57037, CP 60000 Paysandú, Uruguay.; Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Igua 4225, 11400 Montevideo, Uruguay; DILAVE Miguel C. Rubino, Laboratorio Regional Noroeste, Casilla De Correo, 57037, CP 60000 Paysandú, Uruguay.; Departamento de Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, 11500 Montevideo, Uruguay.; Sector de Virología, Universidad Federal de Santa María, Santa Maria, Rio Grande do Sul, Brazil.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, CENUR Litoral Norte Sede Salto, Universidad de la República, Gral. Rivera 1350, 50000 Salto, Uruguay. |
Título : |
An extensive field study reveals the circulation of new genetic variants of subtype 1a of bovine viral diarrhea virus in Uruguay. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Archives of Virology, 1 January 2020, Volume 165, Issue 1, Pages 145-156. Doi: https://doi.org/10.1007/s00705-019-04446-z |
DOI : |
10.1007/s00705-019-04446-z |
Idioma : |
Inglés |
Notas : |
Article history:Received: 22 April 2019 / Accepted: 21 September 2019. |
Contenido : |
Abstract:
Bovine viral diarrhea virus (BVDV) is a major pathogen worldwide, causing signifcant economic losses to the livestock sector. In Uruguay, BVDV seroprevalence at the farm level is >80%. In this work, 2546 serum, blood or tissue samples collected from animals suspected of being afected by BVD between 2015 and 2017 were analyzed by reverse transcription PCR and sequencing. Analysis of the BVDV genomic regions 5?UTR/Npro, Npro and E2 revealed that BVDV-1a, 1i and 2b circulate
in the country, with BVDV-1a being the most prevalent subtype. Population dynamics studies revealed that BVDV-1a has been circulating in our herds since ~1990. This subtype began to spread and evolve, accumulating point mutations at a rate of 3.48 × 10?3 substitutions/site/year, acquiring specifc genetic characteristics that gave rise to two local genetic lineages of BVDV-1a. These lineages are divergent from those circulating worldwide, as well as the vaccine strain currently used
in Uruguay. The most notable diferences between feld and vaccine strains were found in the E2 glycoprotein, suggesting that the amino acid substitutions could result in failure of cross-protection/neutralization after vaccination. This is the frst study that compares Uruguayan BVDV feld and vaccine strains with other BVDV strains from throughout the world. The results obtained in this study will be very useful for developing a suitable immunization program for BVDV in Uruguay by
identifying local feld strains as candidates for vaccine development. MenosAbstract:
Bovine viral diarrhea virus (BVDV) is a major pathogen worldwide, causing signifcant economic losses to the livestock sector. In Uruguay, BVDV seroprevalence at the farm level is >80%. In this work, 2546 serum, blood or tissue samples collected from animals suspected of being afected by BVD between 2015 and 2017 were analyzed by reverse transcription PCR and sequencing. Analysis of the BVDV genomic regions 5?UTR/Npro, Npro and E2 revealed that BVDV-1a, 1i and 2b circulate
in the country, with BVDV-1a being the most prevalent subtype. Population dynamics studies revealed that BVDV-1a has been circulating in our herds since ~1990. This subtype began to spread and evolve, accumulating point mutations at a rate of 3.48 × 10?3 substitutions/site/year, acquiring specifc genetic characteristics that gave rise to two local genetic lineages of BVDV-1a. These lineages are divergent from those circulating worldwide, as well as the vaccine strain currently used
in Uruguay. The most notable diferences between feld and vaccine strains were found in the E2 glycoprotein, suggesting that the amino acid substitutions could result in failure of cross-protection/neutralization after vaccination. This is the frst study that compares Uruguayan BVDV feld and vaccine strains with other BVDV strains from throughout the world. The results obtained in this study will be very useful for developing a suitable immunization program for BVDV in Uruguay by
identifying local feld strains as candida... Presentar Todo |
Palabras claves : |
BOVINE VIRAL DIARRHEA (BVD); PLATAFORMA SALUD ANINMAL. |
Thesagro : |
DIARREA. |
Asunto categoría : |
L73 Enfermedades de los animales |
Marc : |
LEADER 02595naa a2200313 a 4500 001 1060513 005 2020-04-20 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1007/s00705-019-04446-z$2DOI 100 1 $aMAYA, L. 245 $aAn extensive field study reveals the circulation of new genetic variants of subtype 1a of bovine viral diarrhea virus in Uruguay.$h[electronic resource] 260 $c2020 500 $aArticle history:Received: 22 April 2019 / Accepted: 21 September 2019. 520 $aAbstract: Bovine viral diarrhea virus (BVDV) is a major pathogen worldwide, causing signifcant economic losses to the livestock sector. In Uruguay, BVDV seroprevalence at the farm level is >80%. In this work, 2546 serum, blood or tissue samples collected from animals suspected of being afected by BVD between 2015 and 2017 were analyzed by reverse transcription PCR and sequencing. Analysis of the BVDV genomic regions 5?UTR/Npro, Npro and E2 revealed that BVDV-1a, 1i and 2b circulate in the country, with BVDV-1a being the most prevalent subtype. Population dynamics studies revealed that BVDV-1a has been circulating in our herds since ~1990. This subtype began to spread and evolve, accumulating point mutations at a rate of 3.48 × 10?3 substitutions/site/year, acquiring specifc genetic characteristics that gave rise to two local genetic lineages of BVDV-1a. These lineages are divergent from those circulating worldwide, as well as the vaccine strain currently used in Uruguay. The most notable diferences between feld and vaccine strains were found in the E2 glycoprotein, suggesting that the amino acid substitutions could result in failure of cross-protection/neutralization after vaccination. This is the frst study that compares Uruguayan BVDV feld and vaccine strains with other BVDV strains from throughout the world. The results obtained in this study will be very useful for developing a suitable immunization program for BVDV in Uruguay by identifying local feld strains as candidates for vaccine development. 650 $aDIARREA 653 $aBOVINE VIRAL DIARRHEA (BVD) 653 $aPLATAFORMA SALUD ANINMAL 700 1 $aMACÍAS-RIOSECO, M. 700 1 $aSILVEIRA, C.S. 700 1 $aGIANNITTI, F. 700 1 $aCASTELLS, M. 700 1 $aRIVERO, R. 700 1 $aCRISTINA, J. 700 1 $aGIANNEECHINI, E. 700 1 $aPUENTES, R. 700 1 $aFLORES, E.F. 700 1 $aRIET-CORREA, F. 700 1 $aCOLINA, R. 773 $tArchives of Virology, 1 January 2020, Volume 165, Issue 1, Pages 145-156. Doi: https://doi.org/10.1007/s00705-019-04446-z
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
29/05/2019 |
Actualizado : |
11/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
-- - -- |
Autor : |
MONTEVERDE, E.; GUTIERREZ, L.; BLANCO, P.H.; PÉREZ DE VIDA, F.; ROSAS, J.E.; BONNECARRERE, V.; QUERO, G.; MCCOUCH, SUSAN |
Afiliación : |
ELIANA MONTEVERDE, Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University.; LUCÍA GUTIERREZ, Department of Agronomy, University of Wisconsin.; PEDRO HORACIO BLANCO BARRAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO BLAS PEREZ DE VIDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN EDUARDO ROSAS CAISSIOLS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GASTÓN QUERO CORRALLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University. |
Título : |
Integrating molecular markers and environmental covariates to interpret genotype by environment interaction in rice (Oryza sativa L.) grown in subtropical areas. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
G3: GENES, GENOMES, GENETICS May 1, 2019, v.9 (5), p. 1519-1531. OPEN ACCESS. |
DOI : |
10.1534/g3.119.400064 |
Idioma : |
Inglés |
Notas : |
Article history: Manuscript received February 6, 2019 // Accepted for publication March 5, 2019// Published Early Online March 15, 2019.
Supplemental material available at Figshare: https://doi.org/10.25387/g3.7685636 |
Contenido : |
Understanding the genetic and environmental basis of genotype · environment interaction (G·E) is of fundamental importance in plant breeding. If we consider G·E in the context of genotype · year interactions (G·Y), predicting which lines will have stable and superior performance across years is an important challenge for breeders. A better understanding of the factors that contribute to the overall grain yield and quality of rice (Oryza sativa L.) will lay the foundation for developing new breeding and selection strategies for combining high quality, with high yield. In this study, we used molecular marker data and environmental covariates (EC) simultaneously to predict rice yield, milling quality traits and plant height in untested environments (years), using both reaction norm models and partial least squares (PLS), in two rice breeding populations (indica and tropical japonica). We also sought to explain G·E by differential quantitative trait loci (QTL) expression in relation to EC. Our results showed that PLS models trained with both molecular markers and EC gave better prediction accuracies than reaction norm models when predicting future years. We also detected milling quality QTL that showed a differential expression conditional on humidity and solar radiation, providing insight for the main environmental factors affecting milling quality in subtropical and temperate rice growing areas. |
Palabras claves : |
ENVIRONMENTAL COVARIATES; GENOMIC PREDICTIONS; GENOTYPE BY ENVIRONMENT INTERACTION; QTL BY ENVIRONMENT INTERACTION. |
Thesagro : |
ARROZ; FITOMEJORAMIENTO; RICE. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12705/1/Blanco-Genes-Genomes-Genetics-2019.pdf
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Marc : |
LEADER 02649naa a2200313 a 4500 001 1059786 005 2019-10-11 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1534/g3.119.400064$2DOI 100 1 $aMONTEVERDE, E. 245 $aIntegrating molecular markers and environmental covariates to interpret genotype by environment interaction in rice (Oryza sativa L.) grown in subtropical areas.$h[electronic resource] 260 $c2019 500 $aArticle history: Manuscript received February 6, 2019 // Accepted for publication March 5, 2019// Published Early Online March 15, 2019. Supplemental material available at Figshare: https://doi.org/10.25387/g3.7685636 520 $aUnderstanding the genetic and environmental basis of genotype · environment interaction (G·E) is of fundamental importance in plant breeding. If we consider G·E in the context of genotype · year interactions (G·Y), predicting which lines will have stable and superior performance across years is an important challenge for breeders. A better understanding of the factors that contribute to the overall grain yield and quality of rice (Oryza sativa L.) will lay the foundation for developing new breeding and selection strategies for combining high quality, with high yield. In this study, we used molecular marker data and environmental covariates (EC) simultaneously to predict rice yield, milling quality traits and plant height in untested environments (years), using both reaction norm models and partial least squares (PLS), in two rice breeding populations (indica and tropical japonica). We also sought to explain G·E by differential quantitative trait loci (QTL) expression in relation to EC. Our results showed that PLS models trained with both molecular markers and EC gave better prediction accuracies than reaction norm models when predicting future years. We also detected milling quality QTL that showed a differential expression conditional on humidity and solar radiation, providing insight for the main environmental factors affecting milling quality in subtropical and temperate rice growing areas. 650 $aARROZ 650 $aFITOMEJORAMIENTO 650 $aRICE 653 $aENVIRONMENTAL COVARIATES 653 $aGENOMIC PREDICTIONS 653 $aGENOTYPE BY ENVIRONMENT INTERACTION 653 $aQTL BY ENVIRONMENT INTERACTION 700 1 $aGUTIERREZ, L. 700 1 $aBLANCO, P.H. 700 1 $aPÉREZ DE VIDA, F. 700 1 $aROSAS, J.E. 700 1 $aBONNECARRERE, V. 700 1 $aQUERO, G. 700 1 $aMCCOUCH, SUSAN 773 $tG3: GENES, GENOMES, GENETICS May 1, 2019$gv.9 (5), p. 1519-1531. OPEN ACCESS.
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